Subsystem Info

Name:Alanine biosynthesis
Author:OlgaZ
Version:267
Last Modified:/vol/public-pseed/FIGdisk/FIG/Data/Subsystems/Alanine biosynthesis/Backup
Literature
Websites
Description At least three pathways (alanine biosynthesis I, alanine biosynthesis II, and alanine biosynthesis III) contribute to the synthesis of alanine.
Taxonomic distribution for the three pathways are as follows:
alanine biosynthesis I: archaea, eubacteria, and eukaryotes
alanine biosynthesis II: archaea and eukaryotes
alanine biosynthesis III: archaea, eubacteria, and eukaryotes.

=============Variant codes:==============

A - main eubacterial pathway with the key enzyme AtvA ( Valine--pyruvate aminotransferase) – “Alanine biosynthesis I pathway”;

A.120 - Alanine biosynthesis I pathway + alanine biosyntheis III pathway ( cysteine desulfurase) = IlvE + AvtA + IscS

A.123 - Alanine biosynthesis I pathway + alanine biosyntheis III pathway ( cysteine desulfurase) + alanine racemase = IlvE + AvtA + IscS + *AlaR;

A.1234 – All above + AlaB (glutamate-pyruvate aminotransferase pathway (eukaryotic);

B - Alanine biosyntheis III pathway, mediated by cysteine desulfurase (archaea, eubacteria, and eukaryotes) = IscS;

C - Alanine biosyntheis III pathway( cysteine desulfurase) and alanine racemase;
IscS + *AlaR, with IlvE or without

D - Alanine biosynthesis II - glutamate-pyruvate aminotransferase pathway (eukaryotic) = AlaB;
E – just alanine racemase present


Notes Alanine biosynthesis I is established only by existence of the relevant enzymes. Its contribution to alanine synthesis remains speculative because alanine auxotrophs have not yet been isolated. Because alanine but not valine represses AvtA, its primary purpose is probably synthesis of L-alanine.

The alanine biosyntheis III pathway, mediated by cysteine desulfurase activity is required to donate sulfane sulfur for the synthesis of Fe-S clusters, thiamine, thionucleosides in tRNAs, biotin, lipoic acid, and molybdopterin probably contributes only a minor amount of the cell's alanine requirement, as judged by the cell's total requirement for sulfane sulfur.

In many eubacteria, alanine racemase occurs as a homodimer. These subunits constitute a major and minor component. In E coli these genes are denoted as dadX (major) and alr(minor).

Bioinformatic analyses currently recognize the AvtA structure as similar to the class I or class I/II structures of ATs, which represent the most common types of ATs present in C. glutamicum. Nevertheless, AvtA is the only AT with exceptionally high activity toward L-alanine as an amino donor instead of preferably using L-glutamate.

===========REFERENCES:=============

1. Karp, P.D. (2003)The MetaCyc Metabolic Pathway Database in: Metabolic Engineering, B. Kholodenko and H. Westerhoff eds., Horizon Scientific Press.

2. Wang MD, Liu L, Wang BM, Berg CM. Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene.J Bacteriol. 1987 Sep;169(9):4228-34.PMID: 3040683

3. Marienhagen J, Kennerknecht N, Sahm H, Eggeling L. Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum. J Bacteriol. 2005 Nov;187(22):7639-46. PMID: 16267288

4. Mihara H, Esaki N. Bacterial cysteine desulfurases: their function and mechanisms.Appl Microbiol Biotechnol. 2002 Oct;60(1-2):12-23. Epub 2002 Sep 4. Review.PMID: 12382038
Variants
VariantDescription
Classification:Amino Acids and Derivatives
Alanine, serine, and glycine